Part:BBa_K1807001:Experience

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Applications of BBa_K1807001

Figure 1. Graph showing the phosphate concentration within the media for 5 different ppk variants and 1 ppx variant after 6 hours. Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, PKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used in the experiment. One way ANOVA was used and overall there was no statistical difference between strains. Post-hoc LSD was also performed and there was statistical difference between Δppx and BW25113. * signifies there is p< 0.05.(ANOVA: F=1.616; d.f. = 17, p<0.229). Results were measured using a BMG FLUOstar Omega plate reader at OD₆₅₀ and concentrations calculated from known phosphate concentration standards.

Figure 2. Graph showing the phosphate concentration of cell lysate of 10^8, cells for 5 different ppk variants and 1 ppx variant after 6 hours. ; Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, pKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used in the experiment. No statistical difference was shown. Results were measured using a BMG FLUOstar Omega plate reader at OD₆₅₀ and concentrations were calculated from known phosphate concentration standards.

Result

The Ec PPX protein generator does not show significant increase or decrease in phosphate uptake/storage.

User Reviews

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